| Cambridge United FC v Southport 21/08/2010 | View match | |
| Southport v Altrincham 24/08/2010 | View match | |
| Southport v Fleetwood Town FC 28/08/2010 | View match | |
| Forest Green Rovers v Southport 04/09/2010 | View match | |
| Gateshead v Southport 07/09/2010 | View match | |
| Southport v Mansfield Town FC 11/09/2010 | View match | |
| Southport v Rushden & Diamonds 18/09/2010 | View match | |
| Wrexham FC v Southport 21/09/2010 | View match | |
| Darlington FC v Southport 25/09/2010 | View match | |
| Southport v Hayes & Yeading United 28/09/2010 | View match | |
| Southport v Tamworth 02/10/2010 | View match | |
| Histon v Southport 05/10/2010 | View match |