| Southport v Sutton United 22/10/2016 | View match | |
| Southport v York City FC 25/10/2016 | View match | |
| Torquay United FC v Southport 29/10/2016 | View match | |
| Southport v Fleetwood Town FC 07/11/2016 | View match | |
| Southport v Maidstone United (1992) 12/11/2016 | View match | |
| Fleetwood Town FC v Southport 15/11/2016 | View match | |
| Boreham Wood v Southport 19/11/2016 | View match | |
| Chester FC v Southport 22/11/2016 | View match | |
| Southport v Braintree Town 26/11/2016 | View match | |
| Southport v Solihull Moors 29/11/2016 | View match | |
| Southport v Eastleigh 17/12/2016 | View match | |
| Wrexham FC v Southport 26/12/2016 | View match |