| Macclesfield Town FC v Southport 16/08/2016 | View match | |
| Lincoln City FC v Southport 20/08/2016 | View match | |
| Southport v Tranmere Rovers FC 27/08/2016 | View match | |
| Forest Green Rovers v Southport 29/08/2016 | View match | |
| Southport v Dover Athletic FC 03/09/2016 | View match | |
| Eastleigh v Southport 10/09/2016 | View match | |
| Southport v Barrow AFC 13/09/2016 | View match | |
| Southport v Aldershot Town F.C 17/09/2016 | View match | |
| North Ferriby United v Southport 24/09/2016 | View match | |
| Southport v Bromley FC 01/10/2016 | View match | |
| Solihull Moors v Southport 04/10/2016 | View match | |
| Guiseley AFC v Southport 08/10/2016 | View match |