| Southport v Forest Green Rovers 17/02/2001 | View match | |
| Southport v Kettering Town 03/03/2001 | View match | |
| Southport v Chester City FC 13/03/2001 | View match | |
| Southport v Nuneaton Borough 17/03/2001 | View match | |
| Boston United v Southport 25/03/2001 | View match | |
| Southport v Hereford United 27/03/2001 | View match | |
| Southport v Northwich Victoria 31/03/2001 | View match | |
| Southport v Stevenage Borough FC 05/04/2001 | View match | |
| Hayes & Yeading United v Southport 07/04/2001 | View match | |
| Woking FC v Southport 10/04/2001 | View match | |
| Southport v Kingstonian FC 14/04/2001 | View match | |
| Dover Athletic FC v Southport 16/04/2001 | View match |