| Southport v Kingstonian FC 09/12/2000 | View match | |
| Leigh RMI v Southport 16/12/2000 | View match | |
| Stevenage Borough FC v Southport 22/12/2000 | View match | |
| Southport v Morecambe FC 26/12/2000 | View match | |
| Morecambe FC v Southport 01/01/2001 | View match | |
| Southport v Rushden & Diamonds 06/01/2001 | View match | |
| Yeovil Town FC v Southport 20/01/2001 | View match | |
| Scarborough FC v Southport 27/01/2001 | View match | |
| AFC Telford Utd v Southport 10/02/2001 | View match | |
| Southport v Forest Green Rovers 17/02/2001 | View match | |
| Southport v Kettering Town 03/03/2001 | View match | |
| Southport v Chester City FC 13/03/2001 | View match |