| Southport v Woking FC 23/09/2000 | View match | |
| Southport v Boston United 26/09/2000 | View match | |
| Nuneaton Borough v Southport 30/09/2000 | View match | |
| Northwich Victoria v Southport 03/10/2000 | View match | |
| Kingstonian FC v Southport 14/10/2000 | View match | |
| Kettering Town v Southport 21/10/2000 | View match | |
| Southport v Hayes & Yeading United 21/10/2000 | View match | |
| Hednesford Town F.C v Southport 04/11/2000 | View match | |
| Southport v Dover Athletic FC 11/11/2000 | View match | |
| Havant & Waterlooville v Southport 18/11/2000 | View match | |
| Dagenham & Redbridge v Southport 25/11/2000 | View match | |
| Southport v Doncaster Rovers FC 28/11/2000 | View match |