| Southport v Darlington FC 05/09/1959 | View match | |
| Torquay United FC v Southport 09/09/1959 | View match | |
| Southport v Torquay United FC 15/09/1959 | View match | |
| Southport v Barrow 19/09/1959 | View match | |
| Doncaster Rovers FC v Southport 24/09/1959 | View match | |
| Gillingham FC v Southport 26/09/1959 | View match | |
| Southport v Crystal Palace FC 03/10/1959 | View match | |
| Millwall FC v Southport 05/10/1959 | View match | |
| Stockport County FC v Southport 10/10/1959 | View match | |
| Southport v Exeter City FC 17/10/1959 | View match | |
| Watford FC v Southport 24/10/1959 | View match | |
| Southport v Oldham Athletic FC 31/10/1959 | View match |