| Southampton FC v Rochdale FC 22/09/1965 | View match | |
| Rotherham United FC v Southampton FC 25/09/1965 | View match | |
| Southampton FC v Manchester City FC 02/10/1965 | £4.00 | |
| Bolton Wanderers FC v Southampton FC 09/10/1965 | View match | |
| Burnley FC v Southampton FC 13/10/1965 | View match | |
| Southampton FC v Ipswich Town FC 16/10/1965 | View match | |
| Birmingham City FC v Southampton FC 23/10/1965 | £5.00 | |
| Southampton FC v Leyton Orient FC 30/10/1965 | View match | |
| Southampton FC v Leyton Orient FC 30/10/1965 | View match | |
| Middlesbrough FC v Southampton FC 06/11/1965 | View match | |
| Southampton FC v Huddersfield Town FC 13/11/1965 | View match | |
| Crystal Palace FC v Southampton FC 20/11/1965 | View match |