| Brackley Town v Southport 22/02/2025 | View match | |
| Southport v Curzon Ashton 25/02/2025 | View match | |
| Peterborough Sports v Southport 01/03/2025 | View match | |
| Southport v Kings Lynn Town 08/03/2025 | View match | |
| Marine v Southport 11/03/2025 | View match | |
| Rushall Olympic v Southport 15/03/2025 | View match | |
| Southport v Scarborough Athletic 22/03/2025 | View match | |
| Scunthorpe United FC v Southport 29/03/2025 | View match | |
| Southport v Oxford City FC 05/04/2025 | View match | |
| South Shields AFC v Southport 12/04/2025 | View match | |
| Southport v Chester FC 18/04/2025 | View match | |
| Warrington Town v Southport 21/04/2025 | View match |