| Scarborough Athletic v Southport 19/10/2024 | View match | |
| Leamington v Southport 22/10/2024 | View match | |
| Southport v Scunthorpe United FC 26/10/2024 | View match | |
| Oxford City FC v Southport 02/11/2024 | View match | |
| Southport v South Shields AFC 05/11/2024 | View match | |
| Southport v Peterborough Sports 09/11/2024 | View match | |
| Kings Lynn Town v Southport 23/11/2024 | View match | |
| Curzon Ashton v Southport 26/11/2024 | View match | |
| Southport v Marine 30/11/2024 | View match | |
| Southport v Needham Market 14/12/2024 | View match | |
| Kidderminister Harriers v Southport 21/12/2024 | View match | |
| Southport v Warrington Town 26/12/2024 | View match |