| Hyde United v Prescot Cables 22/03/2025 | View match | |
| Lancaster City v Morpeth Town A.F.C. 22/03/2025 | View match | |
| Leek Town v Ashton United 22/03/2025 | View match | |
| Macclesfield Town FC v Bamber Bridge 22/03/2025 | View match | |
| Matlock Town v Blyth Spartans 22/03/2025 | View match | |
| Mickleover FC v Whitby Town 22/03/2025 | View match | |
| Stockton Town v Ilkeston Town 22/03/2025 | View match | |
| Warrington Rylands FC v Worksop Town FC 22/03/2025 | View match | |
| AFC Fylde v Forest Green Rovers 22/03/2025 | View match | |
| Aldershot Town F.C v Ebbsfleet United 22/03/2025 | View match | |
| Altrincham v Barnet FC 22/03/2025 | View match | |
| Braintree Town v York City FC 22/03/2025 | View match |