| Billericay Town v Wingate & Finchley FC 22/03/2025 | View match | |
| Bognor Regis Town v Dartford 22/03/2025 | View match | |
| Cheshunt FC v Folkestone Invicta 22/03/2025 | View match | |
| Cray Wanderers v Bowers & Pitsea 22/03/2025 | View match | |
| Dover Athletic FC v Dulwich Hamlet 22/03/2025 | View match | |
| Hashtag United v Chatham Town 22/03/2025 | View match | |
| Hastings United v Chichester City 22/03/2025 | View match | |
| Horsham v Hendon FC 22/03/2025 | View match | |
| Lewes v Cray Valley PM 22/03/2025 | View match | |
| Potters Bar Town v Canvey Island 22/03/2025 | View match | |
| Whitehawk FC v Carshalton 22/03/2025 | View match | |
| Boreham Wood v Dorking Wanderers 22/03/2025 | View match |