| Bury FC v Port Vale FC 23/09/2000 | View match | |
| Port Vale FC v Wycombe Wanderers FC 30/09/2000 | View match | |
| Peterborough United FC v Port Vale FC 08/10/2000 | View match | |
| Port Vale FC v Colchester United FC 14/10/2000 | View match | |
| Port Vale FC v Northampton Town FC 17/10/2000 | View match | |
| Wigan Athletic FC v Port Vale FC 21/10/2000 | View match | |
| Port Vale FC v Brentford FC 24/10/2000 | View match | |
| Swansea City FC v Port Vale FC 28/10/2000 | View match | |
| Port Vale FC v Millwall FC 04/11/2000 | View match | |
| Canvey Island v Port Vale FC 19/11/2000 | View match | |
| Port Vale FC v Luton Town FC 25/11/2000 | View match | |
| Port Vale FC v Canvey Island 28/11/2000 | View match |