| Port Vale FC v Brentford FC 24/10/2000 | View match | |
| Swansea City FC v Port Vale FC 28/10/2000 | View match | |
| Port Vale FC v Millwall FC 04/11/2000 | View match | |
| Canvey Island v Port Vale FC 19/11/2000 | View match | |
| Port Vale FC v Luton Town FC 25/11/2000 | View match | |
| Port Vale FC v Canvey Island 28/11/2000 | View match | |
| Walsall FC v Port Vale FC 02/12/2000 | View match | |
| Port Vale FC v Bristol City FC 16/12/2000 | View match | |
| Port Vale FC v Rotherham United FC 22/12/2000 | View match | |
| Wrexham FC v Port Vale FC 26/12/2000 | View match | |
| Port Vale FC v Oldham Athletic FC 06/01/2001 | View match | |
| Swindon Town FC v Port Vale FC 13/01/2001 | View match |