| Port Vale FC v Brentford FC 19/09/1959 | View match | |
| Port Vale FC v Barnsley FC 21/09/1959 | View match | |
| Colchester United FC v Port Vale FC 26/09/1959 | View match | |
| Barnsley FC v Port Vale FC 30/09/1959 | View match | |
| Port Vale FC v Southend United FC 03/10/1959 | View match | |
| Port Vale FC v Accrington Stanley (1891) 10/10/1959 | View match | |
| Port Vale FC v Mansfield Town FC 12/10/1959 | View match | |
| Newport County v Port Vale FC 19/10/1959 | View match | |
| Port Vale FC v Chesterfield FC 24/10/1959 | View match | |
| Wrexham FC v Port Vale FC 31/10/1959 | View match | |
| Port Vale FC v York City FC 07/11/1959 | View match | |
| Dorchester Town v Port Vale FC 14/11/1959 | View match |