| Stevenage Borough FC v Wrexham FC 20/10/2009 | View match | |
| Ebbsfleet United v Wrexham FC 31/10/2009 | View match | |
| Kettering Town v Wrexham FC 03/11/2009 | View match | |
| Wrexham FC v Hayes & Yeading United 14/11/2009 | View match | |
| Histon v Wrexham FC 21/11/2009 | View match | |
| Wrexham FC v Kidderminister Harriers 24/11/2009 | View match | |
| Wrexham FC v Swindon Town FC 28/11/2009 | View match | |
| Wrexham FC v Forest Green Rovers 01/12/2009 | View match | |
| York City FC v Wrexham FC 05/12/2009 | View match | |
| Wrexham FC v AFC Wimbledon FC 23/01/2010 | View match | |
| Rushden & Diamonds v Wrexham FC 26/01/2010 | View match | |
| Altrincham v Wrexham FC 30/01/2010 | View match |