| Durham City v Southport 15/10/1927 | View match | |
| Southport v Accrington Stanley (1891) 22/10/1927 | View match | |
| Wigan Borough v Southport 29/10/1927 | View match | |
| Southport v Doncaster Rovers FC 05/11/1927 | View match | |
| Hartlepool United v Southport 12/11/1927 | View match | |
| Southport v Lincoln City FC 19/11/1927 | View match | |
| Denaby United v Southport 26/11/1927 | View match | |
| Southport v Bradford City FC 03/12/1927 | View match | |
| Bradford Park Avenue v Southport 10/12/1927 | View match | |
| Southport v Crewe Alexandra FC 17/12/1927 | View match | |
| Ashington v Southport 24/12/1927 | View match | |
| Southport v Wrexham FC 26/12/1927 | View match |