| Southport v Brighton & Hove Albion FC 30/01/1965 | View match | |
| Southport v Darlington FC 06/02/1965 | View match | |
| Tranmere Rovers FC v Southport 12/02/1965 | View match | |
| Southport v Chester City FC 20/02/1965 | View match | |
| Torquay United FC v Southport 27/02/1965 | View match | |
| Southport v Stockport County FC 06/03/1965 | View match | |
| York City FC v Southport 12/03/1965 | View match | |
| Southport v Lincoln City FC 20/03/1965 | View match | |
| Brighton & Hove Albion FC v Southport 26/03/1965 | View match | |
| Southport v Oxford United FC 03/04/1965 | View match | |
| Hartlepool United v Southport 05/04/1965 | View match | |
| Barrow v Southport 10/04/1965 | View match |