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| Dover Athletic FC v Bowers & Pitsea 08/03/2025 | View match | |
| Hashtag United v Dulwich Hamlet 08/03/2025 | View match | |
| Hastings United v Hendon FC 08/03/2025 | View match | |
| Horsham v Wingate & Finchley FC 08/03/2025 | View match | |
| Lewes v Dartford 08/03/2025 | View match | |
| Potters Bar Town v Cray Valley PM 08/03/2025 | View match | |
| Whitehawk FC v Chatham Town 08/03/2025 | View match | |
| Brackley Town v Alfreton Town 08/03/2025 | View match | |
| Chorley v Chester FC 08/03/2025 | View match | |
| Hereford F.C. v Darlington FC 08/03/2025 | View match | |
| Kidderminister Harriers v Scarborough Athletic 08/03/2025 | View match |