| Weymouth v Aveley FC 22/02/2025 | View match | |
| Worthing FC v Salisbury City 22/02/2025 | View match | |
| Brackley Town v Southport 22/02/2025 | View match | |
| Curzon Ashton v Buxton 22/02/2025 | View match | |
| Darlington FC v Kings Lynn Town 22/02/2025 | View match | |
| Farsley Celtic v Chester FC 22/02/2025 | View match | |
| Hereford F.C. v Alfreton Town 22/02/2025 | View match | |
| Kidderminister Harriers v Spennymoor Town F.C. 22/02/2025 | View match | |
| Oxford City FC v Scunthorpe United FC 22/02/2025 | View match | |
| Radcliffe Borough v Marine 22/02/2025 | View match | |
| Rushall Olympic v Peterborough Sports 22/02/2025 | View match | |
| Scarborough Athletic v Leamington 22/02/2025 | View match |