| Histon v Salisbury City 22/08/2009 | View match | |
| York City FC v Histon 29/08/2009 | View match | |
| Histon v Stevenage Borough FC 31/08/2009 | View match | |
| Chester City FC v Histon 05/09/2009 | View match | |
| Crawley Town FC v Histon 08/09/2009 | View match | |
| Histon v Kidderminister Harriers 12/09/2009 | View match | |
| Hayes & Yeading United v Histon 19/09/2009 | View match | |
| Histon v Ebbsfleet United 22/09/2009 | View match | |
| AFC Wimbledon FC v Histon 26/09/2009 | View match | |
| Histon v Eastbourne Borough 29/09/2009 | View match | |
| Histon v Cambridge United FC 04/10/2009 | View match | |
| Tamworth v Histon 10/10/2009 | View match |