| Gainsborough v South Shields AFC 14/02/2023 | View match | |
| Gainsborough v Belper Town 18/02/2023 | View match | |
| Ashton United v Gainsborough 25/02/2023 | View match | |
| Guiseley AFC v Gainsborough 04/03/2023 | View match | |
| Gainsborough v Warrington Rylands FC 11/03/2023 | View match | |
| Gainsborough v Nantwich Town 18/03/2023 | View match | |
| Gainsborough v Marine 21/03/2023 | View match | |
| Atherton Collieries v Gainsborough 25/03/2023 | View match | |
| Gainsborough v Warrington Town 01/04/2023 | View match | |
| Lancaster City v Gainsborough 08/04/2023 | View match | |
| Gainsborough v Liversedge FC 10/04/2023 | View match | |
| Radcliffe Borough v Gainsborough 15/04/2023 | View match |